山豆根提取物质量标准研究

第 54 卷第 1 期		Vol. 54 No. 1
2024 年 2 月		Feb 2024

所属栏目：医药及中间体

山豆根提取物质量标准研究

田彩燕1，2，3，安巧2，3，张吉2，3，胡占兴2，3∗，郝小江2，3∗ （1. 贵州中医药大学药学院，贵州贵阳 550025；2. 贵州省天然产物研究中心，贵州贵阳 550014；3. 贵州医科大学省部共建药用植物功效与利用国家重点实验室，贵州贵阳 550014）

摘　要：建立山豆根提取物的质量标准。选取乙醇浓度、料液比、回流提取时间及提取次数为影响因素，采用正交实验法优化山豆根提取物提取工艺，并以HPLC法测定山豆根提取物中苦参碱和氧化苦参碱的含量。优化提取工艺为：乙醇浓度60%、料液比1∶20（g∶mL）、回流时间2 h、提取次数3次。HPLC含量测定结果为：苦参碱及氧化苦参碱在选定范围内线性关系良好，加样回收率RSD不高于2%。提取工艺稳定可行，且建立的HPLC含量测定方法简便、快捷。

关键词：山豆根；苦参碱；氧化苦参碱；提取工艺；高效液相色谱法

中图分类号：R284.1　　文献标识码：A　　文章编号：1009-9212（2024）01-0017-04

Study on the Quality Standard of Sophora Tonkinensis Gagnep Extract

TIAN Cai-yan1,2,3, AN Qiao2,3, ZHANG Ji2,3, HU Zhan-xing2,3∗ , HAO Xiao-jiang2,3∗ (1. School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China; 2. Natural Product Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China; 3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang 550014, China)

Abstract：The quality standard of the extract from the Sophora tonkinensis Gagnep was established. Ethanol concentration, solid-liquid ratio, reflux time and extraction times were selected as the influencing factors. Orthogonal experiment was used to select the extraction technology of the Sophora tonkinensis Gagnep, and the content of matrine and oxymatrine in the Sophora tonkinensis Gagnep was determined by HPLC. Orthogonal test results showed that the optimal extraction process was as followings: ethanol concentration (was 60%), solid-liquid ratio was 1∶20 (g∶mL), reflux time was 2.0 h, extraction times were 3 times. HPLC results showed that matrine and oxymatrine had good linear relationship in the selected range, and the recovery RSD was not higher than 2%. The extraction process is stable and feasible, and the established HPLC method is simple and fast.

Key words：Sophora tonkinensis Gagnep; matrine; oxymatrine; extraction technology; HPLC

基金项目：贵州省科学技术厅科技计划项目：新型喹诺里西啶类绿色农药研究与示范（黔科合支撑［2021］一般181），国家自然科学基金委员会地区科学基金项目（32360105）。

作者简介：田彩燕（1998—），女，贵州遵义人，硕士研究生，研究方向：天然药物化学（E-mail：1911500758@qq.com）。

联系人：胡占兴，副研究员，博士，研究方向：天然药物化学（E-mail：huzhxi88@163.com）；郝小江，研究员，博士，研究方向：天然药物化学（E-mail：haoxj@mail.kib.ac.cn）。

收稿日期：2023-11-23